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Probing of the rates of alternating access in LacY with Trp fluorescence

Sugar/H(+) symport by lactose permease (LacY) utilizes an alternating access mechanism in which sugar and H(+) binding sites in the middle of the molecule are alternatively exposed to either side of the membrane by sequential opening and closing of inward- and outward-facing hydrophilic cavities. He...

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Hlavní autoři: Smirnova, Irina, Kasho, Vladimir, Sugihara, Junichi, Kaback, H. Ronald
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2799877/
https://ncbi.nlm.nih.gov/pubmed/19959662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0911434106
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