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Role of protons in sugar binding to LacY

WT lactose permease of Escherichia coli (LacY) reconstituted into proteoliposomes loaded with a pH-sensitive fluorophore exhibits robust uphill H(+) translocation coupled with downhill lactose transport. However, galactoside binding by mutants defective in lactose-induced H(+) translocation is not a...

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Detalhes bibliográficos
Main Authors: Smirnova, Irina, Kasho, Vladimir, Sugihara, Junichi, Vázquez-Ibar, José Luis, Kaback, H. Ronald
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3479522/
https://ncbi.nlm.nih.gov/pubmed/23033496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1214890109
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