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Residues in the H(+) Translocation Site Define the pK(a) for Sugar Binding to LacY
A remarkably high pK(a) of approximately 10.5 has been determined for sugar-binding affinity to the lactose permease of Escherichia coli (LacY), indicating that, under physiological conditions, substrate binds to fully protonated LacY. We have now systematically tested site-directed replacements for...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2009
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2769999/ https://ncbi.nlm.nih.gov/pubmed/19689129 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi9011918 |
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