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Residues in the H(+) Translocation Site Define the pK(a) for Sugar Binding to LacY

A remarkably high pK(a) of approximately 10.5 has been determined for sugar-binding affinity to the lactose permease of Escherichia coli (LacY), indicating that, under physiological conditions, substrate binds to fully protonated LacY. We have now systematically tested site-directed replacements for...

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Detalhes bibliográficos
Main Authors: Smirnova, Irina, Kasho, Vladimir, Sugihara, Junichi, Choe, Jun-Yong, Kaback, H. Ronald
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2769999/
https://ncbi.nlm.nih.gov/pubmed/19689129
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi9011918
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