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Site-directed alkylation and the alternating access model for LacY

In a functional lactose permease mutant from Escherichia coli (LacY) devoid of native Cys residues, almost every residue was replaced individually with Cys and tested for reactivity with the permeant alkylating agent N-ethylmaleimide in right-side-out membrane vesicles. Here we present the results i...

詳細記述

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書誌詳細
主要な著者: Kaback, H. Ronald, Dunten, R., Frillingos, S., Venkatesan, P., Kwaw, I., Zhang, W., Ermolova, Natalia
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 2007
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1766412/
https://ncbi.nlm.nih.gov/pubmed/17172438
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0609968104
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