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Site-directed alkylation of LacY: effect of the proton electrochemical gradient
Previous N-ethylmaleimide-labeling studies show that ligand binding increases the reactivity of single-Cys mutants located predominantly on the periplasmic side of LacY and decreases reactivity of mutants located for the most part on the cytoplasmic side. Thus, sugar binding appears to induce openin...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2224895/ https://ncbi.nlm.nih.gov/pubmed/17920075 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.09.006 |
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