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Site-directed alkylation of LacY: effect of the proton electrochemical gradient
Previous N-ethylmaleimide-labeling studies show that ligand binding increases the reactivity of single-Cys mutants located predominantly on the periplasmic side of LacY and decreases reactivity of mutants located for the most part on the cytoplasmic side. Thus, sugar binding appears to induce openin...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2007
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2224895/ https://ncbi.nlm.nih.gov/pubmed/17920075 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.09.006 |
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