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Site-directed alkylation studies with LacY provide evidence for the alternating access model of transport
Fifty-nine single Cys-replacement mutants in helix VII and helix X of the lactose permease of Escherichia coli were subjected to site-directed fluorescence labeling in right-side-out membrane vesicles to complete the testing of Cys accessibility or reactivity. For both helices, accessibility/reactiv...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3057939/ https://ncbi.nlm.nih.gov/pubmed/21254783 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101988s |
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