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Widespread siRNA “off-target” transcript silencing mediated by seed region sequence complementarity

Transfected siRNAs and miRNAs regulate numerous transcripts that have only limited complementarity to the active strand of the RNA duplex. This process reflects natural target regulation by miRNAs, but is an unintended (“off-target”) consequence of siRNA-mediated silencing. Here we demonstrate that...

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Detalhes bibliográficos
Main Authors: Jackson, Aimee L., Burchard, Julja, Schelter, Janell, Chau, B. Nelson, Cleary, Michele, Lim, Lee, Linsley, Peter S.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1484447/
https://ncbi.nlm.nih.gov/pubmed/16682560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.25706
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