Position-specific chemical modification of siRNAs reduces “off-target” transcript silencing

Transfected siRNAs regulate numerous transcripts sharing limited complementarity to the RNA duplex. This unintended (“off-target”) silencing can hinder the use of RNAi to define gene function. Here we describe position-specific, sequence-independent chemical modifications that reduced silencing of p...

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Bibliographic Details
Main Authors: Jackson, Aimee L., Burchard, Julja, Leake, Devin, Reynolds, Angela, Schelter, Janell, Guo, Jie, Johnson, Jason M., Lim, Lee, Karpilow, Jon, Nichols, Kim, Marshall, William, Khvorova, Anastasia, Linsley, Peter S.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2006
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1484422/
https://ncbi.nlm.nih.gov/pubmed/16682562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.30706
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