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Interactions of mutant and wild-type flap endonucleases with oligonucleotide substrates suggest an alternative model of DNA binding

Previous structural studies on native T5 5′ nuclease, a member of the flap endonuclease family of structure-specific nucleases, demonstrated that this enzyme possesses an unusual helical arch mounted on the enzyme's active site. Based on this structure, the protein's surface charge distrib...

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Bibliographische Detailangaben
Hauptverfasser: Dervan, Joe J., Feng, Min, Patel, Dipak, Grasby, Jane A., Artymiuk, Peter J., Ceska, Thomas A., Sayers, Jon R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2002
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC124304/
https://ncbi.nlm.nih.gov/pubmed/12084915
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.082241699
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