Single copy shRNA configuration for ubiquitous gene knockdown in mice

RNA interference through the expression of small hairpin RNA (shRNA) molecules has become a very promising tool in reverse mouse genetics as it may allow inexpensive and rapid gene function analysis in vivo. However, the prerequisites for ubiquitous and reproducible shRNA expression are not well def...

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Autors principals: Seibler, Jost, Küter-Luks, Birgit, Kern, Heidrun, Streu, Sandra, Plum, Leona, Mauer, Jan, Kühn, Ralf, Brüning, Jens C., Schwenk, Frieder
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2005
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1079974/
https://ncbi.nlm.nih.gov/pubmed/15831785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni065
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