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Single copy shRNA configuration for ubiquitous gene knockdown in mice

RNA interference through the expression of small hairpin RNA (shRNA) molecules has become a very promising tool in reverse mouse genetics as it may allow inexpensive and rapid gene function analysis in vivo. However, the prerequisites for ubiquitous and reproducible shRNA expression are not well def...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Seibler, Jost, Küter-Luks, Birgit, Kern, Heidrun, Streu, Sandra, Plum, Leona, Mauer, Jan, Kühn, Ralf, Brüning, Jens C., Schwenk, Frieder
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2005
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1079974/
https://ncbi.nlm.nih.gov/pubmed/15831785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni065
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