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Single copy shRNA configuration for ubiquitous gene knockdown in mice

RNA interference through the expression of small hairpin RNA (shRNA) molecules has become a very promising tool in reverse mouse genetics as it may allow inexpensive and rapid gene function analysis in vivo. However, the prerequisites for ubiquitous and reproducible shRNA expression are not well def...

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Detalhes bibliográficos
Main Authors: Seibler, Jost, Küter-Luks, Birgit, Kern, Heidrun, Streu, Sandra, Plum, Leona, Mauer, Jan, Kühn, Ralf, Brüning, Jens C., Schwenk, Frieder
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1079974/
https://ncbi.nlm.nih.gov/pubmed/15831785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni065
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