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Inhibiting autophagy by shRNA knockdown: Cautions and recommendations
A glance through Autophagy or any other journal in this field shows that it is very common to block autophagy by RNA interference-based knockdown of ATG mRNAs in mammalian cell lines. Our lab’s experience is that this approach can easily make for failed experiments because good knockdown of even ess...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Landes Bioscience
2013
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3974882/ https://ncbi.nlm.nih.gov/pubmed/23800703 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/auto.24895 |
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