Criteria for effective design, construction, and gene knockdown by shRNA vectors

BACKGROUND: RNA interference (RNAi) technology is a powerful methodology recently developed for the specific knockdown of targeted genes. RNAi is most commonly achieved either transiently by transfection of small interfering (si) RNA oligonucleotides, or stably using short hairpin (sh) RNA expressed...

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Détails bibliographiques
Auteurs principaux: Taxman, Debra J, Livingstone, Laura R, Zhang, Jinghua, Conti, Brian J, Iocca, Heather A, Williams, Kristi L, Lich, John D, Ting, Jenny P-Y, Reed, William
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2006
Sujets:
Methodology Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1409772/
https://ncbi.nlm.nih.gov/pubmed/16433925
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-6-7
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