Temperature-Dependent Function of the Glutamine Phosphoribosylpyrophosphate Amidotransferase Ammonia Channel and Coupling with Glycinamide Ribonucleotide Synthetase in a Hyperthermophile

Genes encoding glutamine phosphoribosylpyrophosphate amidotransferase (GPAT) and glycinamide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in Escherichia coli, and the enzymes were purified to near homogeneity. Both enzymes were maximally active at a temperature of at least 9...

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Hlavní autoři: Bera, Aloke Kumar, Chen, Sihong, Smith, Janet L., Zalkin, Howard
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2000
Témata:
Enzymes and Proteins
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC94544/
https://ncbi.nlm.nih.gov/pubmed/10850988
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