Temperature-Dependent Function of the Glutamine Phosphoribosylpyrophosphate Amidotransferase Ammonia Channel and Coupling with Glycinamide Ribonucleotide Synthetase in a Hyperthermophile

Genes encoding glutamine phosphoribosylpyrophosphate amidotransferase (GPAT) and glycinamide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in Escherichia coli, and the enzymes were purified to near homogeneity. Both enzymes were maximally active at a temperature of at least 9...

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Bibliographic Details
Main Authors: Bera, Aloke Kumar, Chen, Sihong, Smith, Janet L., Zalkin, Howard
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2000
Subjects:
Enzymes and Proteins
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC94544/
https://ncbi.nlm.nih.gov/pubmed/10850988
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