Temperature-Dependent Function of the Glutamine Phosphoribosylpyrophosphate Amidotransferase Ammonia Channel and Coupling with Glycinamide Ribonucleotide Synthetase in a Hyperthermophile

Genes encoding glutamine phosphoribosylpyrophosphate amidotransferase (GPAT) and glycinamide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in Escherichia coli, and the enzymes were purified to near homogeneity. Both enzymes were maximally active at a temperature of at least 9...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Bera, Aloke Kumar, Chen, Sihong, Smith, Janet L., Zalkin, Howard
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Enzymes and Proteins
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10850988/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.13.3734-3739.2000
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