Temperature-Dependent Function of the Glutamine Phosphoribosylpyrophosphate Amidotransferase Ammonia Channel and Coupling with Glycinamide Ribonucleotide Synthetase in a Hyperthermophile

Genes encoding glutamine phosphoribosylpyrophosphate amidotransferase (GPAT) and glycinamide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in Escherichia coli, and the enzymes were purified to near homogeneity. Both enzymes were maximally active at a temperature of at least 9...

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Pubblicato in:	J Bacteriol
Autori principali:	Bera, Aloke Kumar, Chen, Sihong, Smith, Janet L., Zalkin, Howard
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2000
Soggetti:	Enzymes and Proteins
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10850988/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.13.3734-3739.2000
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