Temperature-Dependent Function of the Glutamine Phosphoribosylpyrophosphate Amidotransferase Ammonia Channel and Coupling with Glycinamide Ribonucleotide Synthetase in a Hyperthermophile

Genes encoding glutamine phosphoribosylpyrophosphate amidotransferase (GPAT) and glycinamide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in Escherichia coli, and the enzymes were purified to near homogeneity. Both enzymes were maximally active at a temperature of at least 9...

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שמור ב:
מידע ביבליוגרפי
Main Authors: Bera, Aloke Kumar, Chen, Sihong, Smith, Janet L., Zalkin, Howard
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology 2000
נושאים:
Enzymes and Proteins
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC94544/
https://ncbi.nlm.nih.gov/pubmed/10850988
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