Temperature-Dependent Function of the Glutamine Phosphoribosylpyrophosphate Amidotransferase Ammonia Channel and Coupling with Glycinamide Ribonucleotide Synthetase in a Hyperthermophile

Genes encoding glutamine phosphoribosylpyrophosphate amidotransferase (GPAT) and glycinamide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in Escherichia coli, and the enzymes were purified to near homogeneity. Both enzymes were maximally active at a temperature of at least 9...

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書目詳細資料

發表在:	J Bacteriol
Principais autores:	Bera, Aloke Kumar, Chen, Sihong, Smith, Janet L., Zalkin, Howard
格式:	Artigo
語言:	Inglês
出版:	American Society for Microbiology (ASM) 2000
主題:	Enzymes and Proteins
在線閱讀:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10850988/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.182.13.3734-3739.2000
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