Application of the 5′ Fluorogenic Exonuclease Assay (TaqMan) for Quantitative Ribosomal DNA and rRNA Analysis in Sediments

In this study, we report on the development of quantitative PCR and reverse transcriptase PCR assays for the 16S rRNA of Geobacter spp. and identify key issues related to fluorogenic reporter systems for nucleic acid analyses of sediments. The lower detection limit of each assay was 5 to 50 fg of ge...

Descripció completa

Guardat en:
Dades bibliogràfiques
Autors principals: Stults, Jennie R., Snoeyenbos-West, Oona, Methe, Barbara, Lovley, Derek R., Chandler, Darrell P.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2001
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC92939/
https://ncbi.nlm.nih.gov/pubmed/11375195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.6.2781-2789.2001
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars