Application of the 5′ Fluorogenic Exonuclease Assay (TaqMan) for Quantitative Ribosomal DNA and rRNA Analysis in Sediments

In this study, we report on the development of quantitative PCR and reverse transcriptase PCR assays for the 16S rRNA of Geobacter spp. and identify key issues related to fluorogenic reporter systems for nucleic acid analyses of sediments. The lower detection limit of each assay was 5 to 50 fg of ge...

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Autori principali: Stults, Jennie R., Snoeyenbos-West, Oona, Methe, Barbara, Lovley, Derek R., Chandler, Darrell P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2001
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC92939/
https://ncbi.nlm.nih.gov/pubmed/11375195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.6.2781-2789.2001
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