Application of the 5′ Fluorogenic Exonuclease Assay (TaqMan) for Quantitative Ribosomal DNA and rRNA Analysis in Sediments

In this study, we report on the development of quantitative PCR and reverse transcriptase PCR assays for the 16S rRNA of Geobacter spp. and identify key issues related to fluorogenic reporter systems for nucleic acid analyses of sediments. The lower detection limit of each assay was 5 to 50 fg of ge...

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Bibliografiske detaljer
Main Authors: Stults, Jennie R., Snoeyenbos-West, Oona, Methe, Barbara, Lovley, Derek R., Chandler, Darrell P.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2001
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Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC92939/
https://ncbi.nlm.nih.gov/pubmed/11375195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.67.6.2781-2789.2001
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