Application of the 5′ Fluorogenic Exonuclease Assay (TaqMan) for Quantitative Ribosomal DNA and rRNA Analysis in Sediments

In this study, we report on the development of quantitative PCR and reverse transcriptase PCR assays for the 16S rRNA of Geobacter spp. and identify key issues related to fluorogenic reporter systems for nucleic acid analyses of sediments. The lower detection limit of each assay was 5 to 50 fg of ge...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Stults, Jennie R., Snoeyenbos-West, Oona, Methe, Barbara, Lovley, Derek R., Chandler, Darrell P.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11375195/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.6.2781-2789.2001
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!