Application of the 5′ Fluorogenic Exonuclease Assay (TaqMan) for Quantitative Ribosomal DNA and rRNA Analysis in Sediments

In this study, we report on the development of quantitative PCR and reverse transcriptase PCR assays for the 16S rRNA of Geobacter spp. and identify key issues related to fluorogenic reporter systems for nucleic acid analyses of sediments. The lower detection limit of each assay was 5 to 50 fg of ge...

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書誌詳細

出版年:	Appl Environ Microbiol
主要な著者:	Stults, Jennie R., Snoeyenbos-West, Oona, Methe, Barbara, Lovley, Derek R., Chandler, Darrell P.
フォーマット:	Artigo
言語:	Inglês
出版事項:	American Society for Microbiology (ASM) 2001
主題:	Methods
オンライン･アクセス:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11375195/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.67.6.2781-2789.2001
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