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Protocol for improving diffraction quality of leucyl-tRNA synthetase 1 with methylation and post-crystallization soaking and cooling in cryoprotectants
Leucyl-tRNA synthetase 1 (LARS1) synthesizes Leu-tRNA(Leu) for protein synthesis and plays an important role in mTORC1 activation by sensing intracellular leucine concentrations. Here, we describe a protocol for the purification, reductive methylation, binding affinity measurement by microscale ther...
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| Pubblicato in: | STAR Protoc |
|---|---|
| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8260868/ https://ncbi.nlm.nih.gov/pubmed/34258600 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100642 |
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