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Crystallization of leucyl-tRNA synthetase complexed with tRNA(Leu) from the archaeon Pyrococcus horikoshii
All five tRNA(Leu) isoacceptors from the archaeon Pyrococcus horikoshii have been transcribed in vitro and purified. The leucyl-tRNA synthetase (LeuRS) from P. horikoshii was overexpressed in Escherichia coli and purified, and cocrystallizations with each of the tRNA(Leu) isoacceptors were attempted...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
International Union of Crystallography
2004
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1952391/ https://ncbi.nlm.nih.gov/pubmed/16508082 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104021827 |
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