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Crystallization of leucyl-tRNA synthetase complexed with tRNA(Leu) from the archaeon Pyrococcus horikoshii

All five tRNA(Leu) isoacceptors from the archaeon Pyrococcus horikoshii have been transcribed in vitro and purified. The leucyl-tRNA synthetase (LeuRS) from P. horikoshii was overexpressed in Escherichia coli and purified, and cocrystallizations with each of the tRNA(Leu) isoacceptors were attempted...

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Bibliografiske detaljer
Main Authors: Fukunaga, Ryuya, Ishitani, Ryuichiro, Nureki, Osamu, Yokoyama, Shigeyuki
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2004
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952391/
https://ncbi.nlm.nih.gov/pubmed/16508082
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104021827
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