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Crystallization of leucyl-tRNA synthetase complexed with tRNA(Leu) from the archaeon Pyrococcus horikoshii
All five tRNA(Leu) isoacceptors from the archaeon Pyrococcus horikoshii have been transcribed in vitro and purified. The leucyl-tRNA synthetase (LeuRS) from P. horikoshii was overexpressed in Escherichia coli and purified, and cocrystallizations with each of the tRNA(Leu) isoacceptors were attempted...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
International Union of Crystallography
2004
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1952391/ https://ncbi.nlm.nih.gov/pubmed/16508082 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309104021827 |
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