Protocol for improving diffraction quality of leucyl-tRNA synthetase 1 with methylation and post-crystallization soaking and cooling in cryoprotectants

Leucyl-tRNA synthetase 1 (LARS1) synthesizes Leu-tRNA(Leu) for protein synthesis and plays an important role in mTORC1 activation by sensing intracellular leucine concentrations. Here, we describe a protocol for the purification, reductive methylation, binding affinity measurement by microscale ther...

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Detalles Bibliográficos
Publicado en:STAR Protoc
Autores principales: Kim, Sulhee, Yoon, Ina, Kim, Sunghoon, Hwang, Kwang Yeon
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8260868/
https://ncbi.nlm.nih.gov/pubmed/34258600
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100642
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