The bridge helix of Cas12a imparts selectivity in cis-DNA cleavage and regulates trans-DNA cleavage

Cas12a is an RNA-guided DNA endonuclease of the type V-A CRISPR-Cas system that has evolved convergently with the type II Cas9 protein. We previously showed that proline substitutions in the bridge helix (BH) impart target DNA cleavage selectivity in Streptococcus pyogenes (Spy) Cas9. Here, we exami...

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Detaylı Bibliyografya
Yayımlandı:FEBS Lett
Asıl Yazarlar: Parameshwaran, Hari Priya, Babu, Kesavan, Tran, Christine, Guan, Kevin, Allen, Aleique, Kathiresan, Venkatesan, Qin, Peter Z., Rajan, Rakhi
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2021
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC8044059/
https://ncbi.nlm.nih.gov/pubmed/33523494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/1873-3468.14051
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