Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein

Optogenetic control of CRISPR–Cas9 systems has significantly improved our ability to perform genome perturbations in living cells with high precision in time and space. As new Cas orthologues with advantageous properties are rapidly being discovered and engineered, the need for straightforward strat...

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Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Hoffmann, Mareike D, Mathony, Jan, Upmeier zu Belzen, Julius, Harteveld, Zander, Aschenbrenner, Sabine, Stengl, Christina, Grimm, Dirk, Correia, Bruno E, Eils, Roland, Niopek, Dominik
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2020
Schlagworte:
Methods Online
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7969004/
https://ncbi.nlm.nih.gov/pubmed/33330940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1198
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