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Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity

The limited target specificity of CRISPR-Cas nucleases poses a challenge with respect to their application in research and therapy. Here, we present a simple and original strategy to enhance the specificity of CRISPR-Cas9 genome editing by coupling Cas9 to artificial inhibitory domains. Applying a c...

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Detalhes bibliográficos
Publicado no:Sci Adv
Main Authors: Aschenbrenner, Sabine, Kallenberger, Stefan M., Hoffmann, Mareike D., Huck, Adrian, Eils, Roland, Niopek, Dominik
Formato: Artigo
Idioma:Inglês
Publicado em: American Association for the Advancement of Science 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7002122/
https://ncbi.nlm.nih.gov/pubmed/32076642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.aay0187
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