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An improved method for precise genome editing in zebrafish using CRISPR-Cas9 technique
Current methods of CRISPR-Cas9-mediated site-specific mutagenesis create deletions and small insertions at the target site which are repaired by imprecise non-homologous end-joining. Targeting of the Cas9 nuclease relies on a short guide RNA (gRNA) corresponding to the genome sequence approximately...
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| Publicado no: | Mol Biol Rep |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer Netherlands
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7925485/ https://ncbi.nlm.nih.gov/pubmed/33481178 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11033-020-06125-8 |
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