Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System

We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous zebrafish genes. Here we demonstrate that RGN-in...

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Detalhes bibliográficos
Main Authors: Hwang, Woong Y., Fu, Yanfang, Reyon, Deepak, Maeder, Morgan L., Kaini, Prakriti, Sander, Jeffry D., Joung, J. Keith, Peterson, Randall T., Yeh, Jing-Ruey Joanna
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3706373/
https://ncbi.nlm.nih.gov/pubmed/23874735
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0068708
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