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Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous zebrafish genes. Here we demonstrate that RGN-in...
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Main Authors: | , , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Public Library of Science
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3706373/ https://ncbi.nlm.nih.gov/pubmed/23874735 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0068708 |
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