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Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9
The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5′-NGG-3′ protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureu...
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| Publicado no: | G3 (Bethesda) |
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| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Genetics Society of America
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4978904/ https://ncbi.nlm.nih.gov/pubmed/27317783 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.116.031914 |
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