Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9

The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5′-NGG-3′ protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureu...

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Bibliographic Details
Published in:G3 (Bethesda)
Main Authors: Feng, Yan, Chen, Cheng, Han, Yuxiang, Chen, Zelin, Lu, Xiaochan, Liang, Fang, Li, Song, Qin, Wei, Lin, Shuo
Format: Artigo
Language:Inglês
Published: Genetics Society of America 2016
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Investigations
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4978904/
https://ncbi.nlm.nih.gov/pubmed/27317783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.116.031914
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