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Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction

In mammalian cells, nutrients and growth factors signal through an array of upstream proteins to regulate the mTORC1 growth control pathway. Because the full complement of these proteins has not been systematically identified, we developed a FACS-based CRISPR-Cas9 genetic screening strategy to pinpo...

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Podrobná bibliografie
Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Condon, Kendall J., Orozco, Jose M., Adelmann, Charles H., Spinelli, Jessica B., van der Helm, Pim W., Roberts, Justin M., Kunchok, Tenzin, Sabatini, David M.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7848693/
https://ncbi.nlm.nih.gov/pubmed/33483422
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2022120118
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