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A novel reverse two-hybrid method for the identification of missense mutations that disrupt protein–protein binding
The reverse two-hybrid system is a powerful method to select mutations that disrupt the interaction between two proteins and therefore to identify the residues involved in this interaction. However, the usefulness of this technique has been limited by its relative complexity when compared to the cla...
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| Publicat a: | Sci Rep |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group UK
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7713115/ https://ncbi.nlm.nih.gov/pubmed/33273586 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-77992-1 |
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