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A novel reverse two-hybrid method for the identification of missense mutations that disrupt protein–protein binding

The reverse two-hybrid system is a powerful method to select mutations that disrupt the interaction between two proteins and therefore to identify the residues involved in this interaction. However, the usefulness of this technique has been limited by its relative complexity when compared to the cla...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Vincent, Olivier, Gutierrez-Nogués, Angel, Trejo-Herrero, Adrían, Navas, María-Angeles
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7713115/
https://ncbi.nlm.nih.gov/pubmed/33273586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-77992-1
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