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Enzymatic synthesis of hypermodified DNA polymers for sequence-specific display of four different hydrophobic groups

A set of modified 2′-deoxyribonucleoside triphosphates (dNTPs) bearing a linear or branched alkane, indole or phenyl group linked through ethynyl or alkyl spacer were synthesized and used as substrates for polymerase synthesis of hypermodified DNA by primer extension (PEX). Using the alkyl-linked dN...

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Publicado en:Nucleic Acids Res
Autores principales: Ondruš, Marek, Sýkorová, Veronika, Bednárová, Lucie, Pohl, Radek, Hocek, Michal
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2020
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7708046/
https://ncbi.nlm.nih.gov/pubmed/33152081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa999
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