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Enzymatic synthesis of hypermodified DNA polymers for sequence-specific display of four different hydrophobic groups

A set of modified 2′-deoxyribonucleoside triphosphates (dNTPs) bearing a linear or branched alkane, indole or phenyl group linked through ethynyl or alkyl spacer were synthesized and used as substrates for polymerase synthesis of hypermodified DNA by primer extension (PEX). Using the alkyl-linked dN...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Ondruš, Marek, Sýkorová, Veronika, Bednárová, Lucie, Pohl, Radek, Hocek, Michal
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7708046/
https://ncbi.nlm.nih.gov/pubmed/33152081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa999
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