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2‐Allyl‐ and Propargylamino‐dATPs for Site‐Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA

A series of 2‐alkylamino‐2′‐deoxyadenosine triphosphates (dATP) was prepared and found to be substrates for the Therminator DNA polymerase, which incorporated only one modified nucleotide into the primer. Using a template encoding for two consecutive adenines, conditions were found for incorporation...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Chemistry
Main Authors: Matyašovský, Ján, Pohl, Radek, Hocek, Michal
Format: Artigo
Jezik:Inglês
Izdano: John Wiley and Sons Inc. 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6221035/
https://ncbi.nlm.nih.gov/pubmed/30074286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.201803973
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