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Enzymatic synthesis of hypermodified DNA polymers for sequence-specific display of four different hydrophobic groups

A set of modified 2′-deoxyribonucleoside triphosphates (dNTPs) bearing a linear or branched alkane, indole or phenyl group linked through ethynyl or alkyl spacer were synthesized and used as substrates for polymerase synthesis of hypermodified DNA by primer extension (PEX). Using the alkyl-linked dN...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Ondruš, Marek, Sýkorová, Veronika, Bednárová, Lucie, Pohl, Radek, Hocek, Michal
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7708046/
https://ncbi.nlm.nih.gov/pubmed/33152081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa999
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