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Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically-relevant forces and timescales

The reversible unfolding and refolding of proteins is a regulatory mechanism of tissue elasticity and signalling, used by cells to sense and adapt to extracellular and intracellular mechanical forces. However, most of these proteins exhibit low mechanical stability, posing technical challenges to th...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Nat Protoc
Hauptverfasser: Tapia-Rojo, Rafael, Mora, Marc, Garcia-Manyes, Sergi
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2024
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7616092/
https://ncbi.nlm.nih.gov/pubmed/38467905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-024-00965-5
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