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Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically-relevant forces and timescales

The reversible unfolding and refolding of proteins is a regulatory mechanism of tissue elasticity and signalling, used by cells to sense and adapt to extracellular and intracellular mechanical forces. However, most of these proteins exhibit low mechanical stability, posing technical challenges to th...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Tapia-Rojo, Rafael, Mora, Marc, Garcia-Manyes, Sergi
Format: Artigo
Sprog:Inglês
Udgivet: 2024
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7616092/
https://ncbi.nlm.nih.gov/pubmed/38467905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-024-00965-5
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