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Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically-relevant forces and timescales

The reversible unfolding and refolding of proteins is a regulatory mechanism of tissue elasticity and signalling, used by cells to sense and adapt to extracellular and intracellular mechanical forces. However, most of these proteins exhibit low mechanical stability, posing technical challenges to th...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Tapia-Rojo, Rafael, Mora, Marc, Garcia-Manyes, Sergi
Formato: Artigo
Idioma:Inglês
Publicado em: 2024
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7616092/
https://ncbi.nlm.nih.gov/pubmed/38467905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-024-00965-5
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