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Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically-relevant forces and timescales

The reversible unfolding and refolding of proteins is a regulatory mechanism of tissue elasticity and signalling, used by cells to sense and adapt to extracellular and intracellular mechanical forces. However, most of these proteins exhibit low mechanical stability, posing technical challenges to th...

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Dettagli Bibliografici
Pubblicato in:Nat Protoc
Autori principali: Tapia-Rojo, Rafael, Mora, Marc, Garcia-Manyes, Sergi
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2024
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7616092/
https://ncbi.nlm.nih.gov/pubmed/38467905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-024-00965-5
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