Protein Domain Guided Screen for Sequence Specific and Phosphorothioate-Dependent Restriction Endonucleases

Modification dependent restriction endonucleases (MDREs) restrict modified DNA, typically with limited sequence specificity (∼2–4 bp). Here, we focus on MDREs that have an SRA and/or SBD (sulfur binding domain) fused to an HNH endonuclease domain, cleaving cytosine modified or phosphorothioated (PT)...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Lutz, Thomas, Czapinska, Honorata, Fomenkov, Alexey, Potapov, Vladimir, Heiter, Daniel F., Cao, Bo, Dedon, Peter, Bochtler, Matthias, Xu, Shuang-yong
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7461809/
https://ncbi.nlm.nih.gov/pubmed/33013736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2020.01960
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