Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

BACKGROUND: Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single cells have been proposed as have single-nucl...

Full description

Saved in:
Bibliographic Details
Published in:Genome Biol
Main Authors: Denisenko, Elena, Guo, Belinda B., Jones, Matthew, Hou, Rui, de Kock, Leanne, Lassmann, Timo, Poppe, Daniel, Clément, Olivier, Simmons, Rebecca K., Lister, Ryan, Forrest, Alistair R. R.
Format: Artigo
Language:Inglês
Published: BioMed Central 2020
Subjects:
Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7265231/
https://ncbi.nlm.nih.gov/pubmed/32487174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02048-6
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items