Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

BACKGROUND: Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single cells have been proposed as have single-nucl...

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Detalhes bibliográficos
Publicado no:Genome Biol
Main Authors: Denisenko, Elena, Guo, Belinda B., Jones, Matthew, Hou, Rui, de Kock, Leanne, Lassmann, Timo, Poppe, Daniel, Clément, Olivier, Simmons, Rebecca K., Lister, Ryan, Forrest, Alistair R. R.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
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Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7265231/
https://ncbi.nlm.nih.gov/pubmed/32487174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02048-6
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