Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing

This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the flow sorting of single cells/nuclei into 384-well plates,...

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Bibliografiska uppgifter
I publikationen:STAR Protoc
Huvudupphovsmän: Jaeger, Baptiste N., Yángüez, Emilio, Gesuita, Lorenzo, Denoth-Lippuner, Annina, Kruse, Merit, Karayannis, Theofanis, Jessberger, Sebastian
Materialtyp: Artigo
Språk:Inglês
Publicerad: Elsevier 2020
Ämnen:
Protocol
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC7501729/
https://ncbi.nlm.nih.gov/pubmed/33000004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100081
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