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Miniaturization of Smart-seq2 for Single-Cell and Single-Nucleus RNA Sequencing
This protocol presents a plate-based workflow to perform RNA sequencing analysis of single cells/nuclei using Smart-seq2. We describe (1) the dissociation procedures for cell/nucleus isolation from the mouse brain and human organoids, (2) the flow sorting of single cells/nuclei into 384-well plates,...
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| Pubblicato in: | STAR Protoc |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2020
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7501729/ https://ncbi.nlm.nih.gov/pubmed/33000004 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100081 |
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