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Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows

BACKGROUND: Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. Multiple approaches for optimal sample dissociation and storage of single cells have been proposed as have single-nucl...

詳細記述

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書誌詳細
出版年:Genome Biol
主要な著者: Denisenko, Elena, Guo, Belinda B., Jones, Matthew, Hou, Rui, de Kock, Leanne, Lassmann, Timo, Poppe, Daniel, Clément, Olivier, Simmons, Rebecca K., Lister, Ryan, Forrest, Alistair R. R.
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2020
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7265231/
https://ncbi.nlm.nih.gov/pubmed/32487174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02048-6
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