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In vitro reconstitution assays of microtubule amplification and lattice repair by the microtubule severing enzymes katanin and spastin

Microtubules are non-covalent dynamic polymers essential for the life of all eukaryotic cells. Their dynamic behavior is regulated by a large array of cellular effectors. In vitro microtubule assays have been instrumental in dissecting the mechanism of microtubule associated proteins. In this chapte...

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Bibliographic Details
Published in:Methods Mol Biol
Main Authors: Vemu, Annapurna, Szczesna, Ewa, Roll-Mecak, Antonina
Format: Artigo
Language:Inglês
Published: 2020
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7153568/
https://ncbi.nlm.nih.gov/pubmed/31879896
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-0219-5_3
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